Induction of epithelial to mesenchymal transition in PMC42-LA human breast carcinoma cells by carcinoma-associated fibroblast secreted factors
2007
Effects of Fibroblasts on Breast Cancer Cell Behavior
publication
Evidence: moderate
Author Information
Author(s): Lebret Stephanie C, Newgreen Donald F, Thompson Erik W, Ackland M Leigh
Primary Institution: Deakin University
Hypothesis
CAFs have a greater influence on the migratory ability of PMC42-LA breast cancer cells compared to NMFs.
Conclusion
CAFs significantly enhance the migratory ability of PMC42-LA cells and induce epithelial-mesenchymal transition, while NMFs do not have the same effect.
Supporting Evidence
- CAFs showed a 2.7-fold increase in α-SMA expression compared to NMFs.
- Vimentin expression was significantly increased in PMC42-LA cells cultured with CAFs.
- CAF-conditioned medium caused a significant increase in the migratory ability of PMC42-LA cells.
Takeaway
This study shows that certain cells in the breast can help cancer cells move and change shape, which might make the cancer spread more easily.
Methodology
The study used conditioned media cultures, Western blot analysis, and immunocytochemistry to analyze the effects of NMFs and CAFs on PMC42-LA cells.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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