hnRNP A1 and HPV16: Understanding Their Role in Epithelial Cell Differentiation
Author Information
Author(s): Cheunim Thanaporn, Zhang Jingxin, Milligan Steven G., McPhillips Maria G., Graham Sheila V.
Primary Institution: Institute of Biomedical and Life Sciences, University of Glasgow
Hypothesis
The study investigates the regulation of hnRNP A1 during the differentiation of HPV16-infected epithelial cells.
Conclusion
The study found that hnRNP A1 is up-regulated during the differentiation of HPV16-infected epithelial cells and binds to the HPV16 late regulatory element.
Supporting Evidence
- hnRNP A1 levels increased significantly during the differentiation of HPV16-infected W12E cells.
- hnRNP A1 binds the HPV16 late regulatory element in differentiated cells.
- Changes in hnRNP A1 levels may facilitate alternative splicing of virus late transcripts.
Takeaway
When cells infected with HPV16 mature, they produce more of a protein called hnRNP A1, which helps the virus make its proteins correctly.
Methodology
The study used monolayer and organotypic raft culture systems to analyze the expression of hnRNP A1 and its binding to the HPV16 late regulatory element.
Limitations
The study primarily focuses on specific cell lines and may not fully represent all epithelial cells infected with HPV16.
Participant Demographics
The study utilized W12E cells, a subclone of an HPV16 positive cell line derived from a low-grade cervical lesion, and HaCaT cells, an immortalized human keratinocyte line.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website