Insulin-like growth factor-1 (IGF-1) induces the activation/phosphorylation of Akt kinase and cAMP response element-binding protein (CREB) by activating different signaling pathways in PC12 cells
2006
IGF-1 Activates Akt and CREB in Neuronal Cells
publication
Evidence: moderate
Author Information
Author(s): Zheng Wen-Hua, Quirion Rémi
Primary Institution: Department of Pharmacology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China
Hypothesis
The study investigates how IGF-1 induces the activation of Akt and CREB through different signaling pathways in PC12 cells.
Conclusion
IGF-1 activates Akt and CREB using distinct pathways in PC12 cells.
Supporting Evidence
- IGF-1 induces sustained phosphorylation of Akt and transient phosphorylation of CREB in PC12 cells.
- The phosphorylation of Akt is mediated by the PI3 kinase pathway.
- The phosphorylation of CREB is dependent on the activation of MAPK and p38 MAPK pathways.
- Survival assays indicate that both Akt and CREB contribute to IGF-1 mediated cell survival.
Takeaway
IGF-1 helps brain cells stay alive by turning on two important proteins, Akt and CREB, but it uses different methods for each.
Methodology
PC12 cells were treated with IGF-1, and the phosphorylation of Akt and CREB was evaluated using Western blotting.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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