Transgenic Mouse Model for Monitoring Bmp4 Expression in the Pancreas
Author Information
Author(s): Yasunaga Mayu, Oumi Nao, Osaki Mitsuhiko, Kazuki Yasuhiro, Nakanishi Tomoko, Oshimura Mitsuo, Sato Kenzo
Primary Institution: Tottori University, Yonago, Japan
Hypothesis
The study aims to establish transgenic mice to monitor Bmp4 expression in the pancreas for toxicological screening.
Conclusion
The transgenic mice developed in this study can effectively monitor Bmp4 expression in the pancreas, which responds to fasting and autophagy.
Supporting Evidence
- The bioluminescent signal was detected mainly in the pancreas in three independent lines of transgenic mice.
- Bmp4 expression in the pancreas increased about 3-fold after 24 hours of fasting.
- The transgenic mice allowed for long-term monitoring of Bmp4 expression without sacrificing the animals.
Takeaway
Scientists created special mice that can show how a protein called Bmp4 works in the pancreas, especially when the mice don't eat for a while.
Methodology
Transgenic mice were created using a reporter plasmid to monitor Bmp4 expression through bioluminescence imaging.
Limitations
The study does not address the long-term effects of Bmp4 expression monitoring in the transgenic mice.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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