Role of p53 Serine 46 in Gene Regulation
Author Information
Author(s): Smeenk Leonie, van Heeringen Simon J., Koeppel Max, Gilbert Bianca, Janssen-Megens Eva, Stunnenberg Hendrik G., Lohrum Marion
Primary Institution: Radboud University Nijmegen, Nijmegen, The Netherlands
Hypothesis
How does p53 discriminate between target genes to be activated or repressed in response to cellular stress?
Conclusion
The study shows that p53 phosphorylated at Serine 46 plays a significant role in determining the choice of target genes during apoptosis.
Supporting Evidence
- p53 binding patterns were similar for both treatments but resulted in different gene expression outcomes.
- Phosphorylation at Serine 46 was significantly higher in cells directed towards apoptosis.
- Specific apoptotic target genes showed increased binding of p53 phosphorylated at Serine 46 upon Etoposide treatment.
Takeaway
This study found that a special part of a protein called p53 helps decide which genes to turn on or off when cells are stressed, especially when they are about to die.
Methodology
The researchers treated U2OS cells with Actinomycin D and Etoposide, then analyzed p53 binding and gene expression using ChIP-seq and microarray analysis.
Participant Demographics
U2OS osteosarcoma cells expressing wild type p53
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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