Cytotoxic Effects of Pulp Capping Agents on Stem Cells from Deciduous Teeth
Author Information
Author(s): Nazemi Salman Bahareh, Saburi Ehsan, Gheidari Mahtab Mohammadi, Farsadeghi Mahya, Basir Shabestari Samira
Primary Institution: Zanjan University of Medical Sciences
Hypothesis
This study aimed to compare the cytotoxicity of pulp capping agents against mesenchymal stem cells isolated from human exfoliated deciduous teeth.
Conclusion
The cytotoxicity of MTA, Biodentine, CEM cement, and FS was comparable, decreasing over time and with reduced concentration of biomaterials.
Supporting Evidence
- Biocompatibility increased with reduced concentration of pulp capping agents.
- All tested concentrations showed higher biocompatibility at 72 hours compared to 24 hours.
- No significant difference in cytotoxicity among the tested biomaterials at any time point.
- Cytotoxicity decreased over time and with reduced concentration of biomaterials.
Takeaway
The study tested different dental materials to see how harmful they are to special stem cells from baby teeth, finding that they are generally safe when used properly.
Methodology
In vitro study using MTT assay to assess cytotoxicity of different pulp capping agents on SHEDs over 24 and 72 hours.
Limitations
The study was limited to in vitro conditions and may not fully represent in vivo responses.
Participant Demographics
Mesenchymal stem cells isolated from human exfoliated deciduous teeth.
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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