High-throughput SNP genotyping in pea
Author Information
Author(s): Chrystel Deulvot, Hélène Charrel, Amandine Marty, Françoise Jacquin, Cécile Donnadieu, Isabelle Lejeune-Hénaut, Judith Burstin, Grégoire Aubert
Primary Institution: INRA UMRLEG, Dijon, France
Hypothesis
Can high-throughput SNP genotyping technologies be effectively applied to pea for genetic mapping and diversity studies?
Conclusion
The Illumina GoldenGate assay is effective for high-throughput SNP genotyping in diverse pea germplasm, facilitating genetic mapping and diversity studies.
Supporting Evidence
- The assay successfully genotyped 356 out of 384 SNPs, achieving a 92.7% success rate.
- The genotyping results were consistent with Sanger sequencing data.
- The study identified 37 new gene markers for the pea genetic map.
Takeaway
Scientists used a special test to look at many tiny differences in pea plants' DNA, which helps them understand how these plants can be improved.
Methodology
The study involved designing a 384-SNP set and using the Illumina GoldenGate assay to genotype a collection of pea accessions and a mapping population.
Limitations
The study is limited by the number of SNPs available for pea and the need for more genomic sequence data to increase the SNP set.
Participant Demographics
The study included a mapping population of 91 F6 plants and a germplasm collection of 373 Pisum accessions from various geographical origins.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.01
Digital Object Identifier (DOI)
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