Withaferin A Induces Apoptosis in Human Breast Cancer Cells
Author Information
Author(s): Hahm Eun-Ryeong, Moura Michelle B., Kelley Eric E., Van Houten Bennett, Shiva Sruti, Singh Shivendra V.
Primary Institution: University of Pittsburgh School of Medicine
Hypothesis
The study investigates whether Withaferin A (WA) induces apoptosis in human breast cancer cells through reactive oxygen species (ROS) production.
Conclusion
Withaferin A induces apoptosis in human breast cancer cells by generating reactive oxygen species and inhibiting mitochondrial respiration.
Supporting Evidence
- WA treatment caused a dose-dependent increase in reactive oxygen species in breast cancer cells.
- Stable overexpression of Cu,Zn-SOD reduced WA-induced apoptosis.
- WA treatment inhibited oxidative phosphorylation in breast cancer cells.
Takeaway
Withaferin A, a natural compound, can help kill breast cancer cells by making them produce harmful substances called reactive oxygen species.
Methodology
The study used human breast cancer cell lines MDA-MB-231 and MCF-7 to assess the effects of Withaferin A on apoptosis and mitochondrial function.
Limitations
The study primarily focuses on in vitro models, which may not fully represent in vivo conditions.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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