The Transcriptional Repressor Protein NsrR Senses Nitric Oxide Directly via a [2Fe-2S] Cluster NsrR Is an Iron Sulfur Protein
2008
NsrR Protein Senses Nitric Oxide
publication
Evidence: high
Author Information
Author(s): Tucker Nicholas P., Hicks Matthew G., Clarke Thomas A., Crack Jason C., Chandra Govind, Le Brun Nick E., Dixon Ray, Hutchings Matthew I.
Primary Institution: John Innes Centre, University of East Anglia
Hypothesis
NsrR directly senses nitric oxide through a [2Fe-2S] cluster.
Conclusion
NsrR contains an NO-sensitive [2Fe-2S] cluster that is essential for its DNA binding activity.
Supporting Evidence
- NsrR was purified from Streptomyces coelicolor and shown to contain a [2Fe-2S] cluster.
- Exposure to nitric oxide resulted in the loss of NsrR's DNA binding activity.
- The study provides the first experimental evidence of NsrR's NO-sensing mechanism.
Takeaway
NsrR is a protein that helps bacteria detect nitric oxide, and when it binds to nitric oxide, it can't attach to DNA anymore.
Methodology
The study involved purifying NsrR from Streptomyces coelicolor and using spectroscopy to analyze its properties and interactions with nitric oxide.
Digital Object Identifier (DOI)
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