THE SIGNALING PATHWAY OF SRF-SIRTUIN-2 GENE AND MITOCHONDRIAL FUNCTION
2024
The Role of SIRT2 in Mitochondrial Function
publication
Author Information
Author(s): Zhang Xiaomin, Azhar Gohar, Ameer Fathima, Wei Jeanne Y
Primary Institution: University of Arkansas for Medical Sciences
Hypothesis
The SIRT2 gene expression affects mitochondrial function and is regulated by the serum response factor (SRF).
Conclusion
SIRT2 expression improves mitochondrial function under ischemic conditions.
Supporting Evidence
- Two novel SRF binding sites in the SIRT2 promoter were identified.
- SIRT2 expression increased mitochondrial oxygen consumption rate under ischemic conditions.
- SIRT2 expression reduced glycolysis rate, suggesting improved oxidative phosphorylation.
Takeaway
SIRT2 helps cells use energy better when they are not getting enough oxygen.
Methodology
Bioinformatic tools were used to analyze transcription factor binding sites, and mitochondrial function was measured using a Seahorse XFe96 Analyzer.
Digital Object Identifier (DOI)
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