Using Antibody A7 with Iron Oxide for MRI of Rectal Cancer
Author Information
Author(s): Toma A, Otsuji E, Kuriu Y, Okamoto K, Ichikawa D, Hagiwara A, Ito H, Nishimura T, Yamagishi H
Primary Institution: Kyoto Prefectural University of Medicine
Hypothesis
Can monoclonal antibody A7 coupled to superparamagnetic iron oxide be used as a contrast agent for detecting local recurrence of rectal carcinoma?
Conclusion
Monoclonal antibody A7 coupled to superparamagnetic iron oxide is potentially useful as an MR contrast agent for detecting local recurrence of rectal carcinoma.
Supporting Evidence
- A7-Ferumoxides retained binding activities nearly identical to intact Mab A7.
- Larger amounts of A7-Ferumoxides accumulated in the tumour than normal mouse IgG-Ferumoxides.
- The tumour/blood ratio of A7-Ferumoxides increased in a time-dependent manner.
Takeaway
Researchers created a special contrast agent using an antibody that sticks to rectal cancer cells, helping doctors see if the cancer comes back using MRI.
Methodology
The study involved coupling monoclonal antibody A7 to superparamagnetic iron oxide and testing its accumulation in human colorectal carcinoma xenografts in nude mice.
Limitations
The study was conducted in nude mice, which may not fully represent human responses.
Participant Demographics
Nude mice bearing human colorectal carcinoma xenografts.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website