Impact of receptor clustering on ligand binding
2011
Impact of Receptor Clustering on Ligand Binding
Sample size: 500
publication
Evidence: moderate
Author Information
Author(s): Caré Bertrand R, Soula Hédi A
Primary Institution: Université de Lyon
Hypothesis
How does receptor clustering affect ligand binding kinetics?
Conclusion
Receptor clustering decreases the apparent affinity of ligands for receptors, despite the microscopic affinity remaining constant.
Supporting Evidence
- Clustering decreases the overall binding activity of receptors.
- The apparent affinity of ligands decreases with increased receptor clustering.
- Simulations showed that clustering leads to lower receptor occupation at equilibrium.
Takeaway
When receptors on a cell are grouped together, they don't bind to ligands as well, even though each receptor is still good at binding on its own.
Methodology
A Monte-Carlo algorithm was used to simulate ligand diffusion and binding, comparing different receptor clustering configurations.
Limitations
The study does not account for specific biological interactions between receptors or between receptors and internal signaling proteins.
Digital Object Identifier (DOI)
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