Gene silencing in HIV-1 latency by polycomb repressive group
2011
Gene Silencing in HIV-1 Latency by Polycomb Repressive Group
publication
Evidence: moderate
Author Information
Author(s): Kim Hyeon Guk, Kim Kyung-Chang, Roh Tae-Young, Park Jihwan, Jung Kyung-Min, Lee Joo-Shil, Choi Sang-Yun, Kim Sung Soon, Choi Byeong-Sun
Primary Institution: Korea National Institute of Health
Hypothesis
The study investigates the role of histones and their modifications in HIV-1 latency.
Conclusion
Tri-methylation of histone H3K27 and H2A ubiquitylation via polycomb group proteins may play a crucial role in HIV-1 latency.
Supporting Evidence
- Histone H3K9 and H3K27 acetylations showed no difference in parental and NCHA cells.
- Di- and tri-methylation levels were increased in NCHA cells.
- EED, BMI1, and RING2 were upregulated in NCHA cells.
- Increased ubiquitylation at histone H2A was detected in NCHA cells.
Takeaway
The study found that certain changes in proteins that help control gene activity are important for keeping HIV hidden in cells.
Methodology
The study used RT-PCR, ELISA, western blot, and ChIP-seq to analyze histone modifications in HIV-1 latently infected cells.
Digital Object Identifier (DOI)
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