Genomic Characterization of Probiotic Purple Nonsulfur Bacteria Cereibacter sphaeroides Strains S3W10 and SS15: Implications for Enhanced Shrimp Aquaculture
2024

Genomic Study of Probiotic Bacteria for Shrimp Farming

publication Evidence: high

Author Information

Author(s): Klaysubun Chollachai, Chaichana Nattarika, Suwannasin Sirikan, Singkhamanan Kamonnut, Yaikhan Thunchanok, Kantachote Duangporn, Pomwised Rattanaruji, Wonglapsuwan Monwadee, Surachat Komwit, Gil Rosario

Primary Institution: Prince of Songkla University, Songkhla, Thailand

Hypothesis

This study aimed to determine the draft genomes of two C. sphaeroides strains, S3W10 and SS15, and to conduct a comprehensive comparative genomic analysis.

Conclusion

The genomic analysis of C. sphaeroides strains S3W10 and SS15 reveals their potential as probiotics in aquaculture, enhancing shrimp health and growth while ensuring safety.

Supporting Evidence

  • The genomes of S3W10 and SS15 were characterized to evaluate their probiotic properties and safety for aquaculture use.
  • Both strains contain genes related to stress tolerance, nutrient absorption, and antioxidant activity.
  • Previous studies have shown that incorporating C. sphaeroides into shrimp feed improves digestive enzyme activities.
  • Genomic analysis confirmed the absence of virulence factors and antibiotic-resistance genes in both strains.
  • Strain SS15 is notable for its high protein content and robust production of digestive enzymes.
  • Both strains effectively survive under simulated shrimp gastrointestinal conditions.
  • The presence of various genes related to the production of digestive enzymes and vitamin B12 supports their functional benefits.
  • The study highlights the potential of these strains to enhance sustainability in aquaculture.

Takeaway

Scientists studied two types of bacteria that can help shrimp grow better and stay healthy. They found that these bacteria are safe to use in shrimp farming.

Methodology

The study involved whole-genome sequencing and comparative genomic analysis of two C. sphaeroides strains, focusing on their probiotic properties and safety.

Digital Object Identifier (DOI)

10.3390/life14121691

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