Transcriptomic analysis of grain amaranth (Amaranthus hypochondriacus) using 454 pyrosequencing: comparison with A. tuberculatus, expression profiling in stems and in response to biotic and abiotic stress
2011

Transcriptomic Analysis of Grain Amaranth

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Author Information

Author(s): Délano-Frier John P, Avilés-Arnaut Hamlet, Casarrubias-Castillo Kena, Casique-Arroyo Gabriela, Castrillón-Arbeláez Paula A, Herrera-Estrella Luis, Massange-Sánchez Julio, Martínez-Gallardo Norma A, Parra-Cota Fannie I, Vargas-Ortiz Erandi, Estrada-Hernández María G

Primary Institution: Cinvestav-Unidad Irapuato

Hypothesis

What are the gene expression profiles in grain amaranth in response to biotic and abiotic stress?

Conclusion

The study identified numerous genes in grain amaranth that are induced by stress, which could help in developing stress-resistant plants.

Supporting Evidence

  • A total of 2,700,168 raw reads were obtained from six 454 pyrosequencing runs.
  • 1,971 differentially expressed genes were identified in response to at least one of four stress treatments.
  • Numerous genes were found to be induced in response to (a)biotic stress.
  • The transcriptomic data shared similarity with findings reported in other plant species.
  • Functional GO annotation was performed with all contigs/isotigs that produced significant hits.

Takeaway

Researchers studied how grain amaranth plants react to stress and found many genes that help them survive tough conditions.

Methodology

The study used 454 pyrosequencing to analyze the transcriptome of grain amaranth under various stress conditions.

Limitations

The study primarily focused on specific stress conditions and may not cover all possible environmental stresses.

Statistical Information

P-Value

p<0.0001

Statistical Significance

p<0.0001

Digital Object Identifier (DOI)

10.1186/1471-2164-12-363

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