Macrophage-derived neutrophil chemotactic factor and its role in inhibiting neutrophil migration
Author Information
Author(s): B. M. Tavares-Murta, F. Q. Cunha, M. Dias-Baruffi, M. C. Roque-Barreira, S. H. Ferreira
Primary Institution: Faculty of Medicine of Ribeiro Preto, USP, Brazil
Hypothesis
Could the macrophage-derived neutrophil chemotactic factor (MNCF) be responsible for the inhibitory activity on neutrophil migration?
Conclusion
The study suggests that MNCF accounts for the inhibition of neutrophil migration in vivo by the D-gal fraction.
Supporting Evidence
- The intravenous administration of LPS-stimulated macrophage supernatants inhibits neutrophil migration.
- The D-gal- fraction's inhibitory activity was partially prevented by anti-TNF-a and anti-IL-8 antisera.
- The inhibitory activity was detected in a fraction corresponding to a molecular weight of 54 kDa.
Takeaway
This study found that a protein from macrophages can stop white blood cells called neutrophils from moving to where they are needed in the body.
Methodology
The study involved purifying macrophage supernatants and testing their effects on neutrophil migration in rats.
Participant Demographics
Male Wistar rats weighing 180 to 200 g
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
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