Characterisation of the tryptophan synthase alpha subunit in maize
2008
Characterization of Tryptophan Synthase Alpha Subunit in Maize
publication
Evidence: moderate
Author Information
Author(s): Kriechbaumer Verena, Weigang Linda, Fießelmann Andreas, Letzel Thomas, Frey Monika, Gierl Alfons, Glawischnig Erich
Primary Institution: Technische Universität München
Hypothesis
How is tryptophan synthesized in maize?
Conclusion
Maize forms a tryptophan synthase complex, with ZmTSA functioning as the alpha subunit.
Supporting Evidence
- ZmTSA is localized in plastids, the main site for tryptophan biosynthesis in plants.
- ZmTSA forms a complex with ZmTSB1, enhancing its catalytic efficiency.
- Mass spectrometry identified ZmTSA as the native alpha subunit of the tryptophan synthase complex.
Takeaway
This study found that maize has a special team of proteins that work together to make tryptophan, an important building block for proteins.
Methodology
Functional analysis of TSA homologs, protein purification, and mass spectrometry were used to study the tryptophan synthase complex.
Digital Object Identifier (DOI)
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