The HPV-16 E7 oncoprotein induces centriole multiplication through deregulation of Polo-like kinase 4 expression
2011
HPV-16 E7 Protein Causes Centriole Multiplication
Sample size: 89
publication
Evidence: moderate
Author Information
Author(s): Korzeniewski Nina, Treat Benjamin, Duensing Stefan
Primary Institution: University of Pittsburgh Cancer Institute
Hypothesis
The study investigates how the HPV-16 E7 oncoprotein affects Polo-like kinase 4 expression to induce centriole multiplication.
Conclusion
The HPV-16 E7 oncoprotein induces centriole multiplication by deregulating PLK4 expression.
Supporting Evidence
- HPV-16 E7 oncoprotein expression leads to increased PLK4 mRNA levels.
- Depletion of PLK4 protein impairs centriole overduplication induced by HPV-16 E7.
- Stable expression of HPV-16 E7 resulted in a significant increase in cells with aberrant PLK4 dots at maternal centrioles.
Takeaway
The HPV-16 virus can make cells create too many centrioles, which can lead to cancer. This happens because a protein called PLK4 is not working right.
Methodology
The study used real-time quantitative reverse transcriptase PCR and siRNA to analyze PLK4 expression and centriole duplication in human keratinocytes.
Statistical Information
P-Value
p ≤ 0.002
Statistical Significance
p ≤ 0.002
Digital Object Identifier (DOI)
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