Dre2-Tah18 Complex Controls Mitochondrial Integrity and Cell Death in Yeast
Author Information
Author(s): Vernis Laurence, Facca Céline, Delagoutte Emmanuelle, Soler Nicolas, Chanet Roland, Guiard Bernard, Faye Gérard, Baldacci Giuseppe
Primary Institution: Institut Curie, France
Hypothesis
How does the Dre2-Tah18 complex affect cell death in yeast under oxidative stress?
Conclusion
The study concludes that the Dre2-Tah18 complex plays a critical role in regulating cell death in yeast in response to oxidative stress.
Supporting Evidence
- Mutated tah18 strains showed increased resistance to hydrogen peroxide.
- GFP-Tah18 relocalizes to mitochondria after oxidative stress.
- Dre2 interacts with Tah18 and is essential for cell viability.
- Overexpression of Dre2 can suppress growth defects in tah18 mutants.
- Cell death is dependent on the amount of Tah18 present in the cell.
Takeaway
This study found that a protein complex in yeast helps control cell death when the cells are stressed by hydrogen peroxide.
Methodology
The researchers created thermosensitive mutants and assessed their resistance to hydrogen peroxide, along with examining protein interactions and localization.
Limitations
The study primarily focuses on yeast and may not directly translate to other organisms.
Digital Object Identifier (DOI)
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