Developing Genetic Markers for Sugarcane
Author Information
Author(s): Marconi Thiago G, Costa Estela A, Miranda Hercília R, Mancini Melina C, Cardoso-Silva Cláudio B, Oliveira Karine M, Pinto Luciana R, Mollinari Marcelo, Garcia Antônio AF, Souza Anete P
Primary Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG) - Universidade Estadual de Campinas (UNICAMP)
Hypothesis
The aim of this work was to develop new simple sequence repeat (SSR) markers in sugarcane using the sugarcane expressed sequence tag (SUCEST database).
Conclusion
These EST-SSR markers can be efficiently used for genetic mapping studies of segregating sugarcane populations.
Supporting Evidence
- A total of 365 EST-SSR molecular markers with trinucleotide motifs were developed.
- 287 of the EST-SSRs markers amplified fragments of the expected size and were polymorphic.
- The number of alleles ranged from 2-18, with an average of 6 alleles per locus.
- The average polymorphism information content (PIC) was 0.69.
- The discrimination power was high for the majority of the EST-SSRs, with an average value of 0.80.
Takeaway
Researchers created new markers to help study sugarcane genetics, which can make breeding new sugarcane plants faster and easier.
Methodology
The study involved developing and evaluating 365 EST-SSR molecular markers in 18 sugarcane genotypes.
Limitations
The study's findings may be limited by the genetic diversity of the sugarcane varieties used.
Participant Demographics
The study included 18 sugarcane genotypes, comprising 15 varieties and 3 species.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website