Munc18-bound syntaxin readily forms SNARE complexes with synaptobrevin in native plasma membranes
2006
Munc18-Bound Syntaxin Forms SNARE Complexes
publication
Evidence: high
Author Information
Author(s): Zilly Felipe E, Sørensen Jakob B, Jahn Reinhard, Lang Thorsten
Primary Institution: Max Planck Institute for Biophysical Chemistry, Göttingen, Germany
Hypothesis
Munc18–1 facilitates the formation of fusion-competent SNARE complexes by binding to syntaxin.
Conclusion
Munc18–1 stabilizes a half-closed state of syntaxin 1 that allows for the formation of SNARE complexes necessary for exocytosis.
Supporting Evidence
- Munc18–1 forms a complex with syntaxin that allows SNARE complex formation.
- Displacement of Munc18–1 by synaptobrevin requires the presence of SNAP-25.
- Munc18–1 stabilizes a half-closed state of syntaxin 1.
Takeaway
Munc18–1 helps a protein called syntaxin to connect with other proteins so that cells can release their contents, like a balloon popping.
Methodology
The study used inside-out plasma membranes from PC12 cells to analyze the binding and displacement of Munc18–1 and syntaxin.
Statistical Information
P-Value
p<0.0005
Statistical Significance
p<0.0005
Digital Object Identifier (DOI)
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