Antioxidant and Preventive Effects of Extract from Nymphaea candida Flower on In Vitro Immunological Liver Injury of Rat Primary Hepatocyte Cultures
2011

Antioxidant and Hepatoprotective Effects of Nymphaea candida Extract

Sample size: 6 publication 10 minutes Evidence: high

Author Information

Author(s): Zhao Jun, Liu Tao, Ma Long, Yan Ming, Gu Zhengyi, Huang Yi, Xu Fang, Zhao Yu

Primary Institution: Zhejiang University

Hypothesis

The extract of Nymphaea candida has antioxidant and hepatoprotective effects against immunological liver injury in vitro.

Conclusion

The study found that Nymphaea candida extract significantly protects rat hepatocytes from immunological liver injury and exhibits strong antioxidant properties.

Supporting Evidence

  • Nymphaea candida extract showed significant hepatoprotective effects as evidenced by decreased AST and ALT enzyme activities.
  • The ethyl acetate-soluble fraction exhibited the strongest antioxidant capacity with an IC50 value of 12.6 μg/mL for DPPH.
  • NCE significantly scavenged superoxide anion, hydroxyl radicals, and hydrogen peroxide in a dose-dependent manner.
  • NCE concentrations of 5–200 μg/mL were almost non-toxic to the cells with a TC50 value of 393.65 μg/mL.

Takeaway

This study shows that a flower extract from a plant called Nymphaea candida can help protect the liver and fight off harmful substances.

Methodology

The study used in vitro assays to evaluate the antioxidant activity and hepatoprotective effects of the ethyl acetate extract of Nymphaea candida on primary cultured rat hepatocytes.

Limitations

Further investigations are necessary to verify these activities in vivo.

Participant Demographics

Male Sprague-Dawley rats, 200 ± 20 g.

Statistical Information

P-Value

p<0.01

Statistical Significance

p<0.01

Digital Object Identifier (DOI)

10.1093/ecam/nep003

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