Study of the Aig1l Gene Induced by Electroacupuncture in Mice
Author Information
Author(s): Mika Ohta, Aki Sugano, Shuji Goto, Surini Yusoff, Yushi Hirota, Kotaro Funakoshi, Kenji Miura, Eiichi Maeda, Nobuo Takaoka, Nobuko Sato, Hiroshi Ishizuka, Naoki Arizono, Hisahide Nishio, Yutaka Takaoka
Primary Institution: Kobe University Graduate School of Medicine
Hypothesis
The study aims to identify the full-length cDNA sequence of the Aig1l gene and analyze its expression in response to electroacupuncture.
Conclusion
The Aig1l gene is expressed in various tissues, with the highest expression in the brain, and its expression is significantly induced in skeletal muscle after electroacupuncture treatment.
Supporting Evidence
- The complete cDNA sequence of Aig1l was found to be 6073 bp long.
- All seven tissues analyzed expressed the Aig1l gene.
- Electroacupuncture induced the highest expression of Aig1l in skeletal muscle after 3 hours.
Takeaway
Researchers looked at a gene called Aig1l in mice to see how it reacts to a type of acupuncture. They found that this gene is very active in the brain and gets even more active in muscles after acupuncture.
Methodology
The study used cDNA cloning, northern blot analysis, and real-time quantitative PCR to analyze the Aig1l gene expression in various tissues and after electroacupuncture treatment.
Limitations
The biochemical function of the Aig1l gene is not fully explained, and further investigations are needed.
Participant Demographics
Inbred C57BL/6 male mice, 8 weeks old.
Digital Object Identifier (DOI)
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