Artifactually elevated BUN values on the Boehringer Mannheim-Hitachi 737 and 705 automated chemistry analysers and the development of a kinetic BUN method
1987

New Method for Measuring Blood Urea Nitrogen (BUN)

Sample size: 3 publication Evidence: moderate

Author Information

Author(s): William E. Neeley, Melissa Tyson, Kathleen O'Classen, Mary Gruber

Primary Institution: University of California School of Medicine

Hypothesis

The study investigates the discrepancies in BUN values obtained from two different automated chemistry analysers.

Conclusion

The development of a new kinetic method for BUN analysis eliminated erroneous discrepancies in results from the Hitachi analysers.

Supporting Evidence

  • The study found that the BUN values from the Hitachi 737 and 705 were significantly different for the same patient.
  • A new reagent system was developed to address the discrepancies in BUN measurements.
  • Normal BUN values were obtained using the new method after previously elevated values were recorded.

Takeaway

Doctors found that two machines gave very different results for the same blood test, so they created a new way to test that fixed the problem.

Methodology

The study involved comparing BUN results from different analysers and developing a new reagent system and kinetic method for analysis.

Potential Biases

Potential bias due to the small sample size and specific patient conditions.

Limitations

The exact cause of precipitation in the reagents is unknown, and the study was limited to a small number of patients.

Participant Demographics

One patient was a 73-year-old male; additional patients had different forms of multiple myeloma.

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