Microbiome Analysis of Bladder Tissue Samples
Author Information
Author(s): Enderlin Dominik, Bieri Uwe, Gadient Jana, Morsy Yasser, Scharl Michael, Rüschoff Jan Hendrik, Hefermehl Lukas John, Nikitin Anna, Langenauer Janine, Engeler Daniel Stephan, Förster Beat, Obrecht Fabian, Surber Jonathan, Scherer Thomas Paul, Eberli Daniel, Poyet Cédric
Primary Institution: University Hospital of Zurich
Hypothesis
How does the urinary microbiome impact the response rates of bladder cancer to Bacillus Calmette-Guérin therapy?
Conclusion
Microbiome analysis from both fresh frozen and formalin-fixed paraffin-embedded samples is feasible and could aid in clinical practice.
Supporting Evidence
- Alpha diversity is increased in fresh frozen samples compared to formalin-fixed paraffin-embedded samples.
- No significant differences in alpha diversity were detected between cancerous and non-cancerous tissue.
- Microbiome analysis is feasible in both fresh frozen and formalin-fixed paraffin-embedded samples.
Takeaway
This study looked at how different ways of storing bladder tissue samples affect the bacteria found in them. It found that fresh samples have more variety in bacteria than preserved ones.
Methodology
The study compared microbiome diversity in bladder tissue samples stored as fresh frozen and formalin-fixed paraffin-embedded.
Potential Biases
Factors such as age, gender, and ethnicity were not controlled, which may influence results.
Limitations
The small sample size limits the generalizability of the findings.
Participant Demographics
All three patients were male, aged 68, 71, and 78, with a history of smoking.
Statistical Information
P-Value
0.008
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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