Inhibition of aromatase activity and expression in MCF-7 cells by the chemopreventive retinoid N -(4-hydroxy-phenyl)-retinamide
2000
Inhibition of Aromatase Activity by a Retinoid
publication
Author Information
Author(s): Ciolino H P, Wang T T Y, Sathyamoorthy N
Primary Institution: National Cancer Institute-Frederick Cancer Research and Development Center
Hypothesis
Does the synthetic retinoid N -(4-hydroxyphenyl)-retinamide inhibit aromatase activity and expression in breast cancer cells?
Conclusion
The synthetic retinoid 4-HPR inhibits both the activity and expression of aromatase in breast cancer cells.
Supporting Evidence
- 4-HPR caused a dose-dependent inhibition of aromatase activity in JEG-3 human placental carcinoma cells.
- The K m of the substrate increased and the V max of the reaction decreased in the presence of 4-HPR.
- 4-HPR inhibited both basal and cAMP-induced aromatase activity in intact MCF-7 cells.
- The induction of aromatase mRNA expression in MCF-7 cells by cAMP was inhibited in cells treated with 4-HPR.
Takeaway
A special vitamin-like substance can stop a certain process in breast cancer cells that helps them grow.
Methodology
The study examined the effects of 4-HPR on aromatase activity in both isolated microsomes and intact MCF-7 cells.
Digital Object Identifier (DOI)
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