Transcript Analysis of the Extended Hyp-Operon in Cyanobacteria
Author Information
Author(s): Marie Holmqvist, Pia Lindberg, Åsa Agervald, Karin Stensjö, Peter Lindblad
Primary Institution: Department of Photochemistry and Molecular Science, The Ångström Laboratories, Uppsala University
Hypothesis
The five ORFs upstream of the hyp-genes in Nostoc sp. strain PCC 7120 and Nostoc punctiforme may play a role in hydrogen metabolism.
Conclusion
The five ORFs upstream of the hyp-genes are transcribed as one operon and may form transcripts together with the hyp-genes.
Supporting Evidence
- The five ORFs are located between the uptake hydrogenase structural genes and the maturation protein genes.
- These ORFs are transcribed together with the hyp-genes in both Nostoc strains.
- The expression pattern of the five ORFs is similar to that of known hydrogen metabolism genes.
Takeaway
Scientists studied some genes in cyanobacteria that help them manage hydrogen, and found that these genes work together in a special way.
Methodology
The study involved molecular methods to analyze the transcript levels of specific genes after nitrogen depletion in two cyanobacterial strains.
Digital Object Identifier (DOI)
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