Lactation Defect in MMTV-Cre Transgenic Mice
Author Information
Author(s): Yuan Taichang, Wang Yongping, Pao Lily, Anderson Steve M., Gu Haihua
Primary Institution: University of Colorado School of Medicine
Hypothesis
What is the role of protein tyrosine phosphatase Shp1 in mammary gland development?
Conclusion
MMTV-Cre line A mice exhibit a severe lactation defect that limits their use in studies of mammary gland development.
Supporting Evidence
- Shp1 fl/+;MMTV-Cre and MMTV-Cre female mice displayed a severe lactation defect.
- Pups nursed by Shp1 fl/+;MMTV-Cre dams gained weight poorly compared to controls.
- Histological analyses showed defects in lobuloalveolar expansion in MMTV-Cre mice.
Takeaway
Some mice used in research can't feed their babies properly, which means scientists need to be careful when using them in studies.
Methodology
Mice with floxed Shp1 gene were crossed with MMTV-Cre mice, and mammary gland development was examined using histological and biochemical techniques.
Potential Biases
Potential bias due to the reliance on specific mouse lines that may not represent broader populations.
Limitations
The study primarily focuses on one specific MMTV-Cre line and may not generalize to other lines.
Participant Demographics
Mice were backcrossed onto the FVB background for more than 9 generations.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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