Preventing Acute Deaths in Mice from High Dose Cyclophosphamide
Author Information
Author(s): B.D. Evans, I.E. Smith, R.D. Clutterbuck, J.L. Millar
Primary Institution: Royal Marsden Hospital and Institute of Cancer Research
Hypothesis
Can administering cyclophosphamide in divided doses prevent acute deaths in mice?
Conclusion
Divided dosing of cyclophosphamide significantly reduces acute deaths in mice compared to a single high dose.
Supporting Evidence
- 0/10 mice survived after a single high dose of cyclophosphamide, while 14/15 survived with divided doses.
- Divided doses maintained anti-tumor efficacy in human small cell carcinoma xenografts.
- Three complete remissions were achieved with divided dosing compared to none with a single dose.
Takeaway
Giving a medicine called cyclophosphamide in smaller amounts over time helps mice survive better than giving it all at once.
Methodology
Mice were treated with cyclophosphamide either as a single high dose or in divided doses over 8 to 24 hours, and survival rates were measured.
Limitations
The study primarily involved mice, and the long-term effects of divided dosing on other toxicities were not fully explored.
Participant Demographics
C57BL and CBA/lac mice, including immune-suppressed mice for xenograft studies.
Statistical Information
P-Value
<0.001
Statistical Significance
p<0.001
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