Surface Display of Salmonella Proteins in E. coli and Staphylococcus carnosus
Author Information
Author(s): Nhan Nguyen Thanh, Ernesto Gonzalez, Martin Gustavsson, Hai Truong Nam, Gen Larsson
Primary Institution: Royal Institute of Technology (KTH)
Hypothesis
Can Salmonella antigenic proteins be effectively displayed on the surface of E. coli and Staphylococcus carnosus?
Conclusion
The study suggests that E. coli is a more promising host for the surface expression of Salmonella antigens compared to Staphylococcus carnosus.
Supporting Evidence
- Both SefA and H:gm proteins were successfully translocated to the outer membrane of E. coli.
- SefA was confirmed to be facing the extracellular environment, while H:gm's orientation was uncertain due to proteolytic cleavage.
- Polyclonal antibodies confirmed binding to both proteins, indicating potential for immune response.
Takeaway
The researchers wanted to see if they could make Salmonella proteins show up on the outside of two different bacteria, and they found that one type of bacteria worked better for this than the other.
Methodology
The study involved using E. coli and Staphylococcus carnosus to express Salmonella proteins and analyzing their surface display using various techniques including Western blot and FACS.
Limitations
The study did not conclusively establish the orientation of the H:gm protein on the cell surface due to proteolytic cleavage.
Digital Object Identifier (DOI)
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