Cold-inducible RNA binding protein (CIRP), a novel XTcf-3 specific target gene regulates neural development in Xenopus
2008
CIRP Regulates Neural Development in Xenopus
publication
Evidence: moderate
Author Information
Author(s): Stephanie van Venrooy, Dagmar Fichtner, Martin Kunz, Doris Wedlich, Dietmar Gradl
Primary Institution: Zoologisches Institut II, Universität Karlsruhe (TH), Karlsruhe, Germany
Hypothesis
Is CIRP a specific target gene of XTcf-3 that regulates neural development?
Conclusion
CIRP is essential for proper anterior neural development by stabilizing mRNA.
Supporting Evidence
- CIRP is identified as a novel target gene of XTcf-3.
- Knockdown of XTcf-3 broadens anterior neural tissue.
- Depletion of CIRP leads to reduced mRNA stability and similar neural plate enlargement.
- XTcf-3 and CIRP are co-expressed in neural tissue during development.
- XCIRP is essential for proper neural development.
Takeaway
CIRP helps the brain develop properly by keeping important messages in cells stable.
Methodology
The study used antisense morpholino oligonucleotide injections and in situ hybridization to analyze gene expression.
Digital Object Identifier (DOI)
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