Study of G72 Expression in the Human Brain
Author Information
Author(s): Benzel Isabel, Kew James NC, Viknaraja Ramya, Kelly Fiona, de Belleroche Jacqueline, Hirsch Steven, Sanderson Thirza H, Maycox Peter R
Primary Institution: GlaxoSmithKline
Hypothesis
What is the expression level of G72 mRNA and protein in human brain tissues?
Conclusion
The study found no significant levels of G72 mRNA or protein in various human tissues, suggesting that G72 may not play a role in the pathology of schizophrenia.
Supporting Evidence
- Multiple studies have linked the G72/G30 locus to schizophrenia and bipolar disorder.
- Previous reports suggested G72 might activate D-amino acid oxidase, but this was not confirmed.
- Sensitive detection methods failed to identify G72 mRNA in various human tissues.
- Western blotting did not reveal G72 protein in brain regions where it was expected.
Takeaway
The researchers looked for a protein called G72 in human brains but couldn't find it, which means it might not be important for understanding schizophrenia.
Methodology
The study used northern blotting, RT-PCR, and western blotting to analyze G72 expression in human tissues.
Limitations
The study did not detect G72 expression in any of the tissues tested, which may indicate very low expression levels or issues with detection methods.
Digital Object Identifier (DOI)
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