The Impact of HIV Infection and CD4 Cell Count on the Performance of an Interferon Gamma Release Assay in Patients with Pulmonary Tuberculosis
2009

Impact of HIV and CD4 Count on TB Test Performance

Sample size: 161 publication 10 minutes Evidence: moderate

Author Information

Author(s): Aabye Martine G., Ravn Pernille, PrayGod George, Jeremiah Kidola, Mugomela Apolinary, Jepsen Maria, Faurholt Daniel, Range Nyagosya, Friis Henrik, Changalucha John, Andersen Aase B.

Primary Institution: University of Copenhagen

Hypothesis

How does HIV infection and CD4 cell count affect the sensitivity of the QuantiFERON TB-Gold In-Tube test in patients with pulmonary tuberculosis?

Conclusion

The QFT-IT test is reasonably sensitive for diagnosing active TB in HIV-negative patients, but its performance is limited in HIV-positive patients, especially those with low CD4 counts.

Supporting Evidence

  • The QFT-IT was positive in 74% of patients with culture-confirmed PTB.
  • Sensitivity was higher in HIV-negative patients (81%) compared to HIV-positive patients (65%).
  • Sensitivity increased with higher CD4 cell counts in HIV-positive patients.

Takeaway

This study looked at how well a specific TB test works in people with and without HIV. It found that the test works better in people without HIV.

Methodology

161 patients with culture-confirmed pulmonary tuberculosis were tested for HIV and underwent the QFT-IT test along with CD4 cell count measurement.

Potential Biases

Potential biases may arise from the study's reliance on a single test for TB diagnosis and the exclusion of patients without valid HIV results.

Limitations

The study's findings may not be generalizable to populations outside of the TB- and HIV-endemic settings.

Participant Demographics

The study included 161 patients, with 68 (42%) being HIV-positive; more men participated than women (104 vs. 57).

Statistical Information

P-Value

0.02

Confidence Interval

95% CI: 67–81%

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0004220

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