A Collection of Useful Nuisance Compounds for Bioassay Integrity
Author Information
Author(s): Hu Huabin, Yi Xiangyan, Xue Lian, Baell Jonathan B.
Primary Institution: Monash University
Hypothesis
Understanding the types of nuisance compounds that interfere with bioassays can help prioritize progressable screening hits.
Conclusion
The study presents a set of 103 nuisance compounds that can interfere with assay readouts, which will aid researchers in identifying optimizable hits.
Supporting Evidence
- The study compiles a set of 103 nuisance compounds known to interfere with assay readouts.
- These compounds are made available in an assay-ready format for researchers.
- Understanding nuisance compounds can help improve the quality of high-throughput screening assays.
- Identifying problematic compounds can facilitate better drug discovery processes.
Takeaway
This study created a list of over 100 compounds that can mess up experiments, helping scientists avoid false positives when testing new drugs.
Methodology
The nuisance compounds were gathered from literature searches and previous screening experiences, focusing on those with known PAINS moieties.
Potential Biases
Potential bias in the selection of compounds based on previous experiences and literature.
Limitations
The study does not address all possible nuisance compounds and their effects on every type of assay.
Digital Object Identifier (DOI)
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