Nitric oxide induces apoptosis in a human colonic epithelial cell line
1995
Nitric Oxide Induces Apoptosis in Epithelial Cells
publication
Evidence: moderate
Author Information
Author(s): M. Sandoval, X. Liu, P. D. Olivor, X.-J. Zhang, D. A. Clark, M. J. S. Millorc
Primary Institution: University Medical Center, New Orleans, LA, USA
Hypothesis
Nitric oxide may induce apoptosis in cultured epithelial cells.
Conclusion
Nitric oxide induces apoptosis in T84 epithelial cells in a dose-dependent manner.
Supporting Evidence
- Nitric oxide was shown to induce apoptosis in macrophages and tumor cells.
- DNA fragmentation increased in a dose-dependent manner in response to nitric oxide.
- Cell viability was negatively affected by nitric oxide exposure, indicating that apoptosis preceded cell death.
Takeaway
When certain cells in the gut are exposed to nitric oxide, they can die in a specific way called apoptosis, which is like a programmed cell death.
Methodology
T84 cells were treated with nitric oxide and assessed for DNA fragmentation and cell viability using various assays.
Limitations
The study does not explore the long-term effects of nitric oxide exposure on epithelial cells.
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