New Test for Measuring Factor V in Blood
Author Information
Author(s): Derek Tilley, Irina Levit, John A Samis
Primary Institution: University of Ontario Institute of Technology
Hypothesis
The study aimed to develop a microplate-based assay to measure the functional activity of Factor V in human plasma.
Conclusion
The new FV microplate assay is a simple and effective method for measuring Factor V activity in human plasma.
Supporting Evidence
- The assay is sensitive to approximately 24-80pM FV in normal pooled human reference plasma.
- The FV activity in DIC patient plasmas was functionally less active than in normal pooled human reference plasma.
- The assay allows for simultaneous analysis of multiple samples, which is efficient for clinical settings.
Takeaway
Researchers created a new test that can quickly and easily measure a blood factor called Factor V, which helps with blood clotting.
Methodology
The assay uses a kinetic microplate reader to measure changes in absorbance during fibrin clot formation in human plasma.
Potential Biases
The previous manual assays may have been prone to operator bias.
Limitations
The assay may not provide a quantitative measurement of FV activity based on the extent of clot formation.
Participant Demographics
Nine patients with disseminated intravascular coagulation (DIC) and 15 healthy controls aged 18-22 years.
Digital Object Identifier (DOI)
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