Producing More Enzymes with Bacillus subtilis
Author Information
Author(s): Yomantas Yurgis AV, Abalakina Elena G, Golubeva Lyubov I, Gorbacheva Lyubov Y, Mashko Sergey V
Primary Institution: Ajinomoto-Genetika Research Institute
Hypothesis
Can a plasmid-less Bacillus subtilis strain produce glutamyl-specific protease more efficiently through multi-copy gene integration?
Conclusion
The study developed a method for integrating multiple copies of a gene into the Bacillus subtilis chromosome, resulting in efficient production of glutamyl-specific protease.
Supporting Evidence
- The engineered Bacillus subtilis strain produced approximately 0.5 g/L of secreted glutamyl-specific protease.
- The new method allowed for stable integration of multiple gene copies into the bacterial chromosome.
- The performance of the new strain was comparable to that of a strain carrying the gene on a multi-copy plasmid.
Takeaway
Scientists figured out how to make a type of bacteria produce more of a special enzyme by putting extra copies of the gene for that enzyme into its DNA.
Methodology
The study involved constructing a plasmid-less Bacillus subtilis strain and integrating multiple copies of the mpr gene into its chromosome using double-cross recombination.
Limitations
The efficiency of gene conversion decreased with an increase in the number of integrated cassettes, which could affect strain stability.
Digital Object Identifier (DOI)
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