Modeling Human Sialyltransferases
Author Information
Author(s): Sujatha MS, Balaji Petety V
Primary Institution: Indian Institute of Technology Bombay
Hypothesis
The objective of the current work is to model the 3-D structures of human SiaTs which transfer the sialic acid in α2,3-linkage.
Conclusion
The modeled 3-D structures can be used for docking and other modeling studies and for the rational identification of residues to be mutated to impart desired properties.
Supporting Evidence
- The modeled structures have acceptable stereochemical properties.
- The study illustrates the difficulties in modeling specific proteins of interest.
- The results establish a sequence/structure relationship between sialyltransferases of two distinct families.
Takeaway
Scientists created 3-D models of specific proteins that help in transferring sugar molecules, which can be used to understand how to change these proteins for better functions.
Methodology
The 3-D structures of six human SiaTs were modeled using fold-recognition and comparative modeling methods.
Limitations
The fold-recognition servers could not find a template for certain regions, and some regions were not modeled.
Digital Object Identifier (DOI)
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