Nuclear Factor 90, a cellular dsRNA binding protein inhibits the HIV Rev-export function
2006
NF90ctv Inhibits HIV Rev Function
publication
10 minutes
Evidence: moderate
Author Information
Author(s): Silvio Urcuqui-Inchima, Maria Eugenia Castaño, Danièle Hernandez-Verdun, Georges St-Laurent III, Ajit Kumar
Primary Institution: Grupo de Inmunovirología, Corporación Biogénesis, Universidad de Antioquia
Hypothesis
Does NF90ctv interfere with the HIV Rev-mediated export of viral RNA?
Conclusion
NF90ctv competes with HIV Rev function, inhibiting the export of viral RNA.
Supporting Evidence
- NF90ctv inhibits Rev-mediated RNA transport in HeLa cells.
- Three specific regions of NF90ctv are involved in blocking Rev function.
- NF90ctv competes with Rev for binding to the RRE RNA structure.
Takeaway
NF90ctv is a protein that stops HIV from making copies of itself by blocking a key step in the virus's life cycle.
Methodology
HeLa cells were transfected with constructs expressing NF90ctv and Rev to assess their interaction and effects on RNA export.
Limitations
The study primarily focuses on HeLa cells, which may not fully represent other cell types.
Digital Object Identifier (DOI)
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