Acute Insulin Stimulation Induces Phosphorylation of the Na-Cl Cotransporter in Cultured Distal mpkDCT Cells and Mouse Kidney
2011
Insulin and NCC Phosphorylation in the Kidney
publication
Evidence: high
Author Information
Author(s): Sohara Eisei, Rai Tatemitsu, Yang Sung-Sen, Ohta Akihito, Naito Shotaro, Chiga Motoko, Nomura Naohiro, Lin Shih-Hua, Vandewalle Alain, Ohta Eriko, Sasaki Sei, Uchida Shinichi
Primary Institution: Tokyo Medical and Dental University
Hypothesis
Does insulin regulate NCC phosphorylation in the kidney?
Conclusion
Insulin is a potent regulator of NCC phosphorylation in the kidney, involving WNK4 and SPAK.
Supporting Evidence
- Insulin increased phosphorylation of SPAK and NCC in cultured mpkDCT cells.
- Insulin administration to mice increased phosphorylation of OSR1, SPAK, and NCC in the kidney.
- SPAK is essential for insulin-induced NCC phosphorylation.
- WNK4 is involved in the insulin-induced phosphorylation of NCC.
Takeaway
Insulin helps the kidneys absorb more salt by turning on a special switch called NCC, which is controlled by two helpers named WNK4 and SPAK.
Methodology
The study used cultured mpkDCT cells and mouse models to investigate the effects of insulin on NCC phosphorylation.
Statistical Information
P-Value
p<0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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