Importance of Histidine Residues in Pasteurella multocida Toxin Activity
Author Information
Author(s): Pullinger Gillian D, Lax Alistair J
Primary Institution: King’s College London, Dental Institute, Department of Microbiology
Hypothesis
Histidine residues near the active site of the Pasteurella multocida toxin are critical for its activity.
Conclusion
The study found that mutation of histidine residue H1205 caused a complete loss of activity, indicating its critical role in the function of the Pasteurella multocida toxin.
Supporting Evidence
- Mutation of H1205 caused complete loss of activity, indicating its importance in PMT activity.
- PMTH1205L and PMTH1205Y were completely inactive up to a concentration of 100 ng ml-1.
- PMTH1223Y was around 30 times less active than wild-type toxin.
Takeaway
The researchers looked at specific parts of a toxin and found that changing one part made it completely inactive, showing how important that part is for the toxin to work.
Methodology
The study involved site-directed mutagenesis of histidine residues and assays to measure the activity of the mutant toxins in inducing DNA synthesis in fibroblast cells.
Digital Object Identifier (DOI)
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