APCcdh1 Mediates Degradation of the Oncogenic Rho-GEF Ect2 after Mitosis
2011
APC-Cdh1 Mediates Degradation of the Oncogenic Rho-GEF Ect2 after Mitosis
publication
Evidence: moderate
Author Information
Author(s): Caroline Liot, Laetitia Seguin, Aurélie Siret, Catherine Crouin, Susanne Schmidt, Jacques Bertoglio
Primary Institution: Inserm U749, Institut Gustave Roussy, Villejuif, France
Hypothesis
Ect2 is subject to proteasomal degradation after mitosis, following ubiquitination by the APC/C complex and its co-activator Cdh1.
Conclusion
Ect2 is identified as a cell cycle-regulated protein whose degradation may play an important role in RhoA regulation during mitosis.
Supporting Evidence
- Ect2 levels fluctuate during the cell cycle, peaking during G2/M.
- Ect2 is degraded after mitosis, depending on the ubiquitin ligase activity of the APC/C complex.
- Mutations in Ect2 can stabilize the protein and lead to its oncogenic activity.
- Proper nuclear localization of Ect2 is required for its degradation.
Takeaway
Ect2 is a protein that helps cells divide, and it gets broken down after the cell divides to keep things in order.
Methodology
The study involved western blot analyses and mutagenesis to assess Ect2 degradation and its regulation by APC/C and Cdh1.
Digital Object Identifier (DOI)
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