Herpes Simplex Virus Type 1 Us3 Gene Deletion Influences Toll-like Receptor Responses in Cultured Monocytic Cells
2008
Herpes Simplex Virus Type 1 Us3 Gene Deletion and TLR Responses
publication
Evidence: moderate
Author Information
Author(s): Peri Piritta, Mattila Riikka K, Kantola Helena, Broberg Eeva, Karttunen Heidi S, Waris Matti, Vuorinen Tytti, Hukkanen Veijo
Primary Institution: Department of Virology, University of Turku
Hypothesis
Wild type HSV-1 is able to interfere with TLR3 signaling in infected cells.
Conclusion
The Us3 gene deletion influences TLR responses to HSV-1 in monocytic cell cultures.
Supporting Evidence
- The mRNA expression of TLR3 and type I IFNs were significantly increased in d120, R7041 and HSV-1 (F)-infected U937 cells.
- Activation of IRF-3 was observed in infections with d120 and R7041.
- The intracellular expression of TLR3 and MxA were significantly increased in d120 and R7041-infected cells.
- Both the R7041 and d120 viruses were able to induce apoptosis in U937 cell cultures.
Takeaway
When certain herpes viruses are missing a gene called Us3, they can make the immune system's TLR3 response stronger, which helps fight the virus.
Methodology
U937 human monocytic cells were infected with various HSV-1 strains, and TLR mRNA expression was analyzed using quantitative real-time PCR.
Statistical Information
P-Value
0.009, 0.021, 0.024, 0.033, 0.040
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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