Molecular Mechanisms Involved in Vascular Interactions of the Lyme Disease Pathogen in a Living Host Dissemination of B. burgdorferi
2008

How Lyme Disease Bacteria Spread in the Body

Sample size: 17 publication 10 minutes Evidence: high

Author Information

Author(s): Norman M. Ursula, Moriarty Tara J., Dresser Ashley R., Millen Brandie, Kubes Paul, Chaconas George

Primary Institution: University of Calgary

Hypothesis

What are the mechanisms of vascular interactions during the dissemination of Borrelia burgdorferi in living hosts?

Conclusion

The study found that the initiation of spirochete microvascular interactions is dependent on host ligands and the B. burgdorferi protein BBK32.

Supporting Evidence

  • B. burgdorferi dissemination was observed as a multistage process including tethering, dragging, stationary adhesion, and extravasation.
  • Host fibronectin and glycosaminoglycans were found to be crucial for the initiation of microvascular interactions.
  • Low molecular weight heparin significantly inhibited dragging interactions of B. burgdorferi.

Takeaway

The Lyme disease bacteria use special proteins to stick to blood vessel walls and move through the body, which helps them spread and cause infection.

Methodology

The study used intravital microscopy to visualize the interactions of fluorescent B. burgdorferi with the microvasculature of living mice.

Limitations

The study primarily focused on a single strain of B. burgdorferi and may not represent all strains or conditions.

Participant Demographics

Mice were used as the model organism for the study.

Statistical Information

P-Value

0.797

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.ppat.1000169

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