Daily Sampling of an HIV-1 Patient with Slowly Progressing Disease Displays Persistence of Multiple env Subpopulations Consistent with Neutrality
2011

HIV-1 Evolution in a Patient with Slowly Progressing Disease

Sample size: 77 publication 10 minutes Evidence: moderate

Author Information

Author(s): Skar Helena, Gutenkunst Ryan N., Wilbe Ramsay Karin, Alaeus Annette, Albert Jan, Leitner Thomas

Primary Institution: Department of Virology, Swedish Institute for Infectious Disease Control, Solna, Sweden

Hypothesis

How do HIV-1 subpopulation frequencies change over time in a treatment-naive patient?

Conclusion

HIV-1 subpopulation frequencies changed significantly over 1.5 years, but this did not imply directional or balancing selection.

Supporting Evidence

  • 77 sequences of HIV-1 env were obtained from plasma samples.
  • Phylogenetic analysis showed that the sequences clustered in six distinct subpopulations.
  • Subpopulation frequencies remained constant over short time periods but fluctuated significantly over longer periods.

Takeaway

The study looked at how different groups of HIV-1 viruses changed in a patient over time, finding that while they changed a lot, they didn't seem to be competing to be the best.

Methodology

The study involved intensive sampling of HIV-1 sequences from a patient over a 32-day period and additional time points before and after.

Potential Biases

Potential biases may arise from the limited sampling and the specific patient characteristics.

Limitations

The study was limited by the small number of sequences obtained at certain time points.

Participant Demographics

The participant was a treatment-naive, asymptomatic male who had been HIV-1 infected for approximately 7 years.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0021747

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