The induction of CCN2 by TGFβ1 involves Ets-1
2006
How Ets-1 Regulates CCN2 Expression in Fibroblasts
publication
10 minutes
Evidence: moderate
Author Information
Author(s): Van Beek Jonathan P, Kennedy Laura, Rockel Jason S, Bernier Suzanne M, Leask Andrew
Primary Institution: Schulich School of Medicine and Dentistry, The University of Western Ontario
Hypothesis
The expression of CCN2 can be regulated through the activity of Ets-1.
Conclusion
Ets-1 is required for the TGFβ induction of CCN2 expression in fibroblasts.
Supporting Evidence
- Ets-1 binds to the CCN2 promoter and is necessary for its activation by TGFβ.
- Dominant negative Ets-1 reduces CCN2 expression in response to TGFβ.
- Smad3 and Ets-1 work together to enhance CCN2 promoter activity.
Takeaway
Ets-1 helps control a protein called CCN2 that is important for healing and can be involved in diseases like arthritis and cancer.
Methodology
NIH 3T3 fibroblasts were transfected with CCN2 promoter constructs and treated with TGFβ1 to assess promoter activity.
Limitations
The study primarily focuses on fibroblasts and may not generalize to other cell types.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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