A combined functional and structural genomics approach identified an EST-SSR marker with complete linkage to the Ligon lintless-2 genetic locus in cotton (Gossypium hirsutum L.)
2011

Identifying a Genetic Marker Linked to Cotton Fiber Length

Sample size: 136 publication 10 minutes Evidence: moderate

Author Information

Author(s): Hinchliffe Doug J, Turley Rickie B, Naoumkina Marina, Kim Hee Jin, Tang Yuhong, Yeater Kathleen M, Li Ping, Fang David D

Primary Institution: USDA-ARS, Southern Regional Research Center

Hypothesis

The study aims to identify genes involved in fiber elongation in cotton and convert gene expression data into molecular markers.

Conclusion

The research successfully identified an EST-SSR marker with complete linkage to the Li2 locus, suggesting it may be responsible for the short fiber phenotype in cotton.

Supporting Evidence

  • Five SSR markers were closely mapped around the Li2 locus region.
  • Gene expression profiling suggested roles of reactive oxygen species homeostasis and cytokinin regulation in the Li2 mutant phenotype.
  • The EST-derived SSR marker NAU3991 displayed complete linkage to the Li2 locus on chromosome 18.

Takeaway

Scientists studied cotton plants to find a gene that controls how long the cotton fibers grow, and they found a special marker that helps identify this gene.

Methodology

The study involved developing near-isogenic lines of cotton, mapping the Li2 locus using SSR markers, and conducting microarray gene expression analysis.

Limitations

The study lacks empirical evidence for the functionality of the identified candidate genes.

Statistical Information

P-Value

p<0.05

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1186/1471-2164-12-445

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