How Shear Stress Affects ACE Regulation
Author Information
Author(s): Barauna Valerio Garrone, Campos Luciene Cristina Gastalho, Miyakawa Ayumi Aurea, Krieger Jose Eduardo
Primary Institution: Heart Institute (InCor), University of Sao Paulo Medical School, Sao Paulo, Brazil
Hypothesis
We tested whether angiotensin converting enzyme (ACE) and phosphorylation of Ser1270 are involved in shear-stress (SS)-induced downregulation of the enzyme.
Conclusion
ACE plays a key role in its own downregulation in response to shear stress, requiring both its extra- and intracellular domains and phosphorylation at Ser1270.
Supporting Evidence
- Shear stress decreased ACE expression and phosphorylation in human primary endothelial cells.
- ACE phosphorylation on Ser1270 decreased significantly in response to shear stress.
- The study demonstrated that both the extra- and intracellular domains of ACE are required for shear stress-induced downregulation.
Takeaway
When blood flows over blood vessels, it can change how a protein called ACE works. This study shows that ACE can sense this flow and adjust itself accordingly.
Methodology
The study used Western blotting analysis and CHO cell models to investigate ACE expression and phosphorylation in response to shear stress.
Limitations
The study primarily used a cell model system, which may not fully replicate in vivo conditions.
Participant Demographics
Human primary endothelial cells and CHO cells were used in the experiments.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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