Study of ABCG2 in Prostate Cells
Author Information
Author(s): Pascal Laura E, Oudes Asa J, Petersen Timothy W, Goo Young Ah, Walashek Laura S, True Lawrence D, Liu Alvin Y
Primary Institution: University of Washington
Hypothesis
Are the side population (SP) of normal human prostate and ABCG2+ cells similar populations that express putative stem cell markers?
Conclusion
The study provides gene expression profiles for prostate SP and ABCG2+ cells, which are important for understanding prostate development and cancer.
Supporting Evidence
- ABCG2 positive cells were localized to the prostate basal epithelium and endothelium.
- SP cells constituted 0.5–3% of the total epithelial fraction.
- The SP transcriptome was essentially the same as ABCG2+ cells, indicating shared stem cell characteristics.
Takeaway
The researchers looked at special cells in the prostate that might be like stem cells, and found that they share some important features.
Methodology
The study used flow cytometry to isolate prostate cells and analyzed gene expression through DNA array analysis and RT-PCR.
Potential Biases
Potential contamination of stem cells with endothelial cells due to the sorting methods used.
Limitations
The sorting methods resulted in very small numbers of cells that were insufficient for in vitro studies.
Participant Demographics
Cancer-free prostate tissue samples from 30 radical prostatectomies.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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