Differential Recognition of Phytophthora sojae Effectors in Soybean
Author Information
Author(s): Dong Suo, Yu Dan, Cui Linkai, Qutob Dinah, Tedman-Jones Jennifer, Kale Shiv D., Tyler Brett M., Wang Yuanchao, Gijzen Mark
Primary Institution: Agriculture and Agri-Food Canada
Hypothesis
The Avr3a and Avr5 genes of Phytophthora sojae are alleles of a single genetic locus.
Conclusion
The study confirms that Avr3a and Avr5 are alleles and represent a single genetic locus, Avr3a/5, which determines strain-specific virulence in soybean.
Supporting Evidence
- Avr3a and Avr5 co-segregate in 100 F2 progeny.
- Over-expression of Avr3a/5 in a virulent strain results in avirulence to Rps3a and Rps5.
- Silencing of Avr3a/5 causes gain of virulence in a normally avirulent strain.
Takeaway
This study shows that two genes from a plant pathogen can be recognized differently by soybean plants, which helps in understanding how to protect crops from diseases.
Methodology
The study involved genetic mapping, haplotype analysis, and functional tests using transient expression in soybean and stable transformation of Phytophthora sojae.
Limitations
The study does not explore all possible strains of Phytophthora sojae and their interactions with soybean resistance genes.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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